Catalog No: OKBB00559
Size:96 Wells
Price: $583.00
SKU
OKBB00559
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Lumican ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Lumican has been precoated onto 96-well plates. Standards(NS0, Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Lumican amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)143343276957172446837234
SD80.25268.3313.1115.5332.5419.6
CV%5.66.24.56.77.15.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Lumican Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Lumican Standard2 x 10 ng/tube
100X Biotinylated Anti-Human Lumican Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human Lumican
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: Q19-N338
DescriptionFor quantitative detection of human Lumican in cell culture supernatants, serum and plasma(heparin).
Gene SymbolLUM
Gene Full Namelumican
Alias SymbolsKeratan sulfate proteoglycan lumican, KSPG Lumican, LDC, LUM, LUM_HUMAN, Lumican, Lumican precursor, SLRR2D
NCBI Gene Id4060
Protein NameLumican
Description of TargetLumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
Uniprot IDP51884
Protein Accession #NP_002336.1
Nucleotide Accession #NM_002345.3
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