LTF Chemi-Luminescent ELISA Kit (Human) (OKCD03731)

Catalog No: OKCD03731

Size:96WELLS

Human LTF ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-97%	92-105%	92-102%	96-105%
  EDTA Plasma (n=5)	94-102%	92-99%	80-101%	90-101%
  Heparin Plasma (n=5)	86-93%	84-103%	79-97%	78-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoferrin (LTF) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-103	101
  EDTA Plasma (n=5)	83-94	90
  Heparin Plasma (n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LTF Microplate: 96 Wells (12 x 8 Well strips)
  • LTF Lyophilized Standard: 2
  • 100X LTF Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates, breast milk and other biological fluids
• Sensitivity: 0.0549 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LTF
• Gene Full Name: lactotransferrin
• Alias Symbols: epididymis luminal protein 110;GIG12;growth-inhibiting protein 12;HEL110;HLF2;kaliocin-1;lactoferricin;lactoferroxin;lactotransferrin;LF;neutrophil lactoferrin;talalactoferrin.
• NCBI Gene Id: 4057
• Protein Name: Lactotransferrin
• Description of Target: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
• Uniprot ID: P02788
• Protein Accession #: NP_001186078
• Nucleotide Accession #: NM_001199149