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LTF Antibody (OALA03063)

50 ug
$539.00
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Gene Symbol:
LTF
NCBI Gene Id:
4057
Swissprot Id:
P02788
Protein Accession #:
NP_002334.2
Nucleotide Accession #:
NM_002343
Alias Symbols:
LTF, GIG12, Growth-inhibiting protein 12, HLF2, Lactotransferrin, Lactoferrin, LF, Neutrophil lactoferrin, Talalactoferrin
Replacement Item:
This antibody may replace item sc-121273 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Purification:
Immunoaffinity purified
Application:
IHC-P, WB, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LTF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LTF.
Immunogen:
LF / LTF / Lactoferrin antibody was raised against synthetic peptide C-ENYKSQQSSDPDP from an internal region of human LTF / Lactoferrin (NP_002334.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (92%); Monkey (85%).
Predicted Species Reactivity:
Human, Gorilla
Predicted Homology Based on Immunogen Sequence:
Gibbon (at least 90% immunogen sequence identity)
Product Format:
Liquid
Reconstitution and Storage:
Store at -20C. Minimize freezing and thawing.
Concentration:
0.5 mg/ml
Datasheets/Manuals:
Printable datasheet for OALA03063
Specificity:
LTF / Lactoferrin.
Epitope:
Internal
Warning:
For research use only.
Application Info:
IHC - Paraffin (2 ug/ml), Western blot (0.05 - 0.2 ug/ml), ELISA (1:32000)
Application Data:
Immunohistochemistry: OALA03063 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA03063 was determined to be 2 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 85kD band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kD according to NP_002334.2). Recommended concentration: 0.05-0.2 ug/ml.
Storage Buffer:
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

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