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Catalog No: OKEH06257
Size:96T
Price: $625.00
SKU
OKEH06257
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ltc4s ELISA Kit (Rat) (OKEH06257) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ltc4s has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ltc4s is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ltc4s captured in the well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <5.2%, n = 20
Mean Inter-Assay Precision - <9.1%, n = 20
ELISA Kit Component
ComponentAmount
LTC4S Microplate96 Wells (12 x 8 Well strips)
LTC4S Lyophilized Standard2
100X Biotinylated LTC4S Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.63 pg/mL
SpecificityNatural and recombinant Rat Leukotriene C4 synthase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLtc4s
Gene Full Nameleukotriene C4 synthase
Alias Symbolsglutathione S-transferase LTC4;leukotriene C4 synthase;leukotriene-C(4) synthase;LTC4 synthase.
NCBI Gene Id114097
Protein NameQ925U2
Description of TargetLeukotriene C4 synthase
Uniprot IDhttps://www.uniprot.org/uniprot/Q925U2
Protein Accession #NP_446091.2
Nucleotide Accession #NM_053639.2
Protein Size (# AA)150
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