LTB Chemi-Luminescent ELISA Kit (Rat) (OKCD03726)

Catalog No: OKCD03726

Size:96WELLS

Rat LTB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-89%	86-102%	96-104%	88-95%
  EDTA Plasma (n=5)	81-93%	84-102%	87-103%	92-105%
  Heparin Plasma (n=5)	95-103%	99-105%	96-103%	94-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Lymphotoxin Beta (LTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lymphotoxin Beta (LTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lymphotoxin Beta (LTb) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-95	91
  EDTA Plasma (n=5)	79-102	95
  Heparin Plasma (n=5)	81-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphotoxin Beta (LTb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphotoxin Beta (LTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ltb Microplate: 96 Wells (12 x 8 Well strips)
  • Ltb Lyophilized Standard: 2
  • 100X Ltb Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LOC103694381|Ltb
• Gene Full Name: lymphotoxin beta|lymphotoxin-beta
• Alias Symbols: lymphotoxin B;lymphotoxin beta (TNF superfamily, member 3);lymphotoxin-beta.
• NCBI Gene Id: 103694381|361795
• Protein Name: Lymphotoxin B
• Description of Target: Lymphotoxin B
• Uniprot ID: Q6MG45
• Protein Accession #: NP_997672
• Nucleotide Accession #: NM_212507