LTA ELISA Kit (Pig) (OKCD02941)

Catalog No: OKCD02941

Size:96WELLS

Pig LTA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-105%	86-94%	99-105%	91-105%
  EDTA Plasma (n=5)	84-99%	96-105%	94-105%	80-103%
  Heparin Plasma (n=5)	98-105%	91-99%	99-105%	79-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Beta (TNFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Beta (TNFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Beta (TNFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Beta (TNFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-89	81
  EDTA Plasma (n=5)	95-103	98
  Heparin Plasma(n=5)	96-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LTA Microplate: 96 Wells (12 x 8 Well strips)
  • LTA Lyophilized Standard: 2
  • 100X Biotinylated LTA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 5.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Beta (TNFb). No significant cross-reactivity or interference between Tumor Necrosis Factor Beta (TNFb) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LTA
• Gene Full Name: lymphotoxin alpha
• Alias Symbols: LT-alpha;lymphotoxin alpha (TNF superfamily, member 1);lymphotoxin-alpha;TNFB;TNF-beta;tumor necrosis factor ligand superfamily member 1;tumour necrosis factor beta.
• NCBI Gene Id: 407742
• Protein Name: Lymphotoxin-alpha
• Description of Target: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
• Uniprot ID: P26445
• Protein Accession #: NP_999618.1
• Nucleotide Accession #: NM_214453.1
• Protein Size (# AA): 204