Catalog No: OKEH06087
Size:96 Wells
Price: $525.00
SKU
OKEH06087
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LSS ELISA Kit (Rat) (OKEH06087)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Lss ELISA Kit (Rat) (OKEH06087) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lss has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lss is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lss captured in well.
ELISA Kit Range0.312 - 20nmol/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.9%, n = 20
Mean Inter-Assay Precision - <7.7%, n = 20
ELISA Kit Component
ComponentAmount
LSS Microplate96 Wells (12 x 8 Well strips)
LSS Lyophilized Standard2
100X Biotinylated LSS Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156nmol/mL
SpecificityNatural and recombinant Rat Lanosterol synthase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLSS
Gene Full NameLanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Alias Symbols2,3-epoxysqualene--lanosterol cyclase, Lanosterol synthase, Osc, OSC, Oxidosqualene--lanosterol cyclase
NCBI Gene Id81681
Protein NameLanosterol synthase
Description of TargetCatalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency.
Uniprot IDP48450
Protein Accession #NP_112311.1
Nucleotide Accession #NM_031049.1
Protein Size (# AA)733
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