Catalog No: OKCD08525
Size:96WELLS
Price: $825.00
SKU
OKCD08525
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for LSR ELISA Kit (Mouse) (OKCD08525) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipolysis Stimulated Lipoprotein Receptor (LSR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipolysis Stimulated Lipoprotein Receptor (LSR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipolysis Stimulated Lipoprotein Receptor (LSR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipolysis Stimulated Lipoprotein Receptor (LSR) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipolysis Stimulated Lipoprotein Receptor (LSR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipolysis Stimulated Lipoprotein Receptor (LSR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.059ng/mL |
Gene Symbol | Lsr |
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Gene Full Name | lipolysis stimulated lipoprotein receptor |
Alias Symbols | IL;ILDR3;lipolysis stimulated receptor;lipolysis-stimulated lipoprotein receptor;Lipolysis-stimulated receptor;Lis;Lisch7;liver-specific bHLH-Zip transcription factor;liver-specific gene on mouse chromosome 7 protein. |
NCBI Gene Id | 54135 |
Protein Name | Lipolysis-stimulated lipoprotein receptor |
Description of Target | Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity). |
Uniprot ID | Q99KG5 |
Protein Accession # | NP_001157656.1 |
Nucleotide Accession # | NM_001164184.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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