LSR ELISA Kit (Human) (OKCD00406)

Catalog No: OKCD00406 (Formerly GWB-KBBAZ9)

Size:96WELLS

Human LSR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipolysis Stimulated Lipoprotein Receptor (LSR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipolysis Stimulated Lipoprotein Receptor (LSR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipolysis Stimulated Lipoprotein Receptor (LSR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipolysis Stimulated Lipoprotein Receptor (LSR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipolysis Stimulated Lipoprotein Receptor (LSR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipolysis Stimulated Lipoprotein Receptor (LSR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LSR Microplate: 96 Wells (12 x 8 Well Strips)
  • LSR Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated LSR Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipolysis Stimulated Lipoprotein Receptor (LSR). No significant cross-reactivity or interference between Lipolysis Stimulated Lipoprotein Receptor (LSR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LSR
• Gene Full Name: lipolysis stimulated lipoprotein receptor
• Alias Symbols: angulin-1;ILDR3;immunoglobulin-like domain containing receptor 3;lipolysis-stimulated lipoprotein receptor;lipolysis-stimulated remnant;LISCH protein;LISCH7;liver-specific bHLH-Zip transcription factor.
• NCBI Gene Id: 51599
• Protein Name: Lipolysis-stimulated lipoprotein receptor
• Description of Target: Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity).
• Uniprot ID: Q86X29
• Protein Accession #: NP_001247418
• Nucleotide Accession #: NM_001260489.1
• Protein Size (# AA): 649