LRRFIP1 ELISA Kit (Human) (OKCD01038)

Catalog No: OKCD01038 (Formerly GWB-KBBYQ2)

Size:96WELLS

Human LRRFIP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LRRFIP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • LRRFIP1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated LRRFIP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). No significant cross-reactivity or interference between Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LRRFIP1
• Gene Full Name: LRR binding FLII interacting protein 1
• Alias Symbols: FLAP1;FLAP-1;FLIIAP1;GC-binding factor 2;GCF2;GCF-2;HUFI-1;leucine rich repeat (in FLII) interacting protein 1;leucine-rich repeat flightless-interacting protein 1;LRR FLII-interacting protein 1;NEDD8-conjugating enzyme;TAR RNA-interacting protein;TRIP.
• NCBI Gene Id: 9208
• Protein Name: Leucine-rich repeat flightless-interacting protein 1
• Description of Target: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.
• Uniprot ID: Q32MZ4
• Protein Accession #: NP_001131022
• Nucleotide Accession #: NM_001137550.1
• Protein Size (# AA): 808