LRRC4B ELISA Kit (Human) (OKCD02118)

Catalog No: OKCD02118 (Formerly GWB-KBBYX7)

Size:96 Wells

Human LRRC4B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology LRRC4B ELISA Kit (Human) (OKCD02118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRRC4B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LRRC4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LRRC4B captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level LRRC4B was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level LRRC4B was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-LRRC4B Microplate: 96 Wells (12 x 8 Well Strips)
  • LRRC4B Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated LRRC4B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.133 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing Protein 4B (LRRC4B). No significant cross-reactivity or interference between Leucine Rich Repeat Containing Protein 4B (LRRC4B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LRRC4B
• Gene Full Name: Leucine rich repeat containing 4B
• Alias Symbols: HSM, LRIG4, NGL-3
• NCBI Gene Id: 94030
• Protein Name: Leucine-rich repeat-containing protein 4B
• Description of Target: Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner.
• Uniprot ID: Q9NT99
• Protein Accession #: NP_001073926.1
• Nucleotide Accession #: NM_001080457.1
• Protein Size (# AA): 713