Catalog No: OKEH04600
Size:96T
Price: $525.00
SKU
OKEH04600
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology LRP5 ELISA Kit (Human) (OKEH04600) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRP5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LRP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRP5 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-LRP5 Microplate96 Wells (12 x 8 Well Strips)
LRP5 Lyophilized Standard2
Sample Diluent1 x 20 mL
LRP5 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.19 ng/mL
SpecificityNatural and recombinant Human Low-density lipoprotein receptor-related protein 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLRP5
Gene Full NameLDL receptor related protein 5
Alias SymbolsBMND1;EVR1;EVR4;HBM;low density lipoprotein receptor-related protein 5;low density lipoprotein receptor-related protein 7;low-density lipoprotein receptor-related protein 5;LR3;LRP-5;LRP7;LRP-7;OPPG;OPS;OPTA1;PCLD4;VBCH2.
NCBI Gene Id4041
Protein NameO75197
Description of TargetLow-density lipoprotein receptor-related protein 5
Uniprot IDhttps://www.uniprot.org/uniprot/O75197
Protein Accession #NP_001278831.1
Nucleotide Accession #NM_001291902.1
Protein Size (# AA)1615
Molecular Weight179 KDa
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