Catalog No: OKEH00004
Size:96T
Price: $525.00
SKU
OKEH00004
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LRP2 ELISA Kit (Rat) : 96 Wells (OKEH00004)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LRP2 ELISA Kit (Rat) (OKEH00004) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LRP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRP2 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
LRP2 Microplate96 Wells (12 x 8 Well strips)
LRP2 Lyophilized Standard2
100X Biotinylated LRP2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.312 ng/mL
SpecificityNatural and recombinant Rat Low-density lipoprotein receptor-related protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLrp2
Gene Full NameLDL receptor related protein 2
Alias Symbolsglycoprotein 330;gp330;low density lipoprotein-related protein 2;low-density lipoprotein receptor-related protein 2;LRP-2;megalin.
NCBI Gene Id29216
Protein NameP98158
Description of TargetLow-density lipoprotein receptor-related protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/P98158
Protein Accession #NP_110454
Nucleotide Accession #NM_030827
Protein Size (# AA)4660
Molecular Weight519 kDa
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