Catalog No: OKEH01469
Price: $600.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01469
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LRP1 ELISA Kit (Mouse) (OKEH01469) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LRP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
LRP1 Microplate96 Wells (12 x 8 Well strips)
LRP1 Lyophilized Standard2
100X Biotinylated LRP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.093 ng/mL
SpecificityNatural and recombinant Mouse Prolow-density lipoprotein receptor-related protein 1

Macrophage cells secrete factors including LRP1 that orchestrate the rejuvenation of bone repair in mice. Nat Commun. 9, 5191 (2018). 30518764

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLrp1
Gene Full Namelow density lipoprotein receptor-related protein 1
Alias SymbolsA2;A2mr;AI316852;alpha 2-macroglobulin receptor;Alpha-2-macroglobulin receptor;b2b1554C;b2b1554Clo;CD91;lipoprotein receptor-related protein;Lrp;LRP-1;prolow-density lipoprotein receptor-related protein 1.
NCBI Gene Id16971
Protein NameQ91ZX7
Description of TargetProlow-density lipoprotein receptor-related protein 1
Uniprot ID
Protein Accession #NP_032538.2
Nucleotide Accession #NM_008512.2
Protein Size (# AA)4545
Molecular Weight505 kDa
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