LRP1 ELISA Kit (Human) (OKCD07054)

Catalog No: OKCD07054

Size:96WELLS

LRP1 ELISA Kit (Human) (OKCD07054) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 1 (LRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 1 (LRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LRP1 Microplate: 96 Wells (12 x 8 Well strips)
  • LRP1 Lyophilized Standard: 2
  • 100X LRP1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 28.4pg/mL

Target Info

• Gene Symbol: LRP1
• Gene Full Name: LDL receptor related protein 1
• Alias Symbols: A2MR;alpha-2-macroglobulin receptor;APOER;apolipoprotein E receptor;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;low density lipoprotein receptor-related protein 1;LRP;LRP1A;prolow-density lipoprotein receptor-related protein 1;TbetaR-V/LRP-1/IGFBP-3 receptor;TGFBR5;type V tgf-beta receptor.
• NCBI Gene Id: 4035
• Protein Name: Prolow-density lipoprotein receptor-related protein 1
• Description of Target: Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PubMed:11907044, PubMed:12888553, PubMed:12713657). Acts as an alpha-2-macroglobulin receptor (PubMed:26142438).
• Uniprot ID: Q07954
• Protein Accession #: NP_002323.2
• Nucleotide Accession #: NM_002332.2