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LRP1 Antibody (OASA02540)

1 mg
$1,350.00
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Predicted Species Reactivity:
Human
Product Format:
Purified IgG - liquid
Clonality:
Monoclonal
Clone:
A2Mr alpha-2
Isotype:
IgG1
Host:
Mouse
Conjugation:
Azide Free
Application:
IHC-AFF, ELISA, FC, IP, WB
Additional Information:
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Histology Positive Control Tissue: Liver
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: None present
:::
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage:
Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Symbol:
LRP1
NCBI Gene Id:
4035
Protein Name:
Prolow-density lipoprotein receptor-related protein 1
Swissprot Id:
Q07954
Protein Accession #:
NP_002323.2
Alias Symbols:
APR, LRP, A2MR, CD91, APOER, TGFBR5, IGFBP3R, FLJ16451, MGC88725, LRP1
Replacement Item:
This antibody may replace item sc-16166 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD91:Azide Free
Protein Size (# AA):
4544
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LRP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LRP1.
Immunogen:
The immunogen for anti-LRP1 antibody: purified alpha2 macroglobulin receptor
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for anti-LRP1 antibody - OASA02540
Specificity:

Mouse anti Human CD91 antibody, clone A2Mr alpha­2 recognizes human CD91, also known as Prolow­density lipoprotein receptor­related protein 1, Alpha­2­macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85kDa type I transmembrane carboxyl chain (LRP85) non­covalently bound to a 515kDa extracellular N­terminal subunit (LRP515)containing multiple EGF­like and LDL­-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha­2 detects an epitope within the LRP515 chain. 


CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated
serum alpha­2­macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992). 
Mouse anti Human CD91, clone A2Mr alpha­2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009). 

Application Info:
Flow Cytometry: 1/50 1/100
ELISA: Direct
Western Blotting(1): Non-reducing conditions
Target Reference:
1. Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues. Cell Tissue Res. 269: 375-382.
2. Moestrup, S.K. and Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells. Leuk. Res. 16: 227-234.
3. Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression. J Biol Chem. 285: 25458-66.

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