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Catalog No: OKCD01614 (Formerly GWB-KBBOU9)
Size:96WELLS
Price: $650.00
SKU
OKCD01614
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Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-105%85-94%97-104%97-104%
EDTA Plasma (n=5)97-105%81-104%87-103%86-102%
Heparin Plasma (n=5)88-96%89-99%78-91%80-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Alpha-2-Glycoprotein 1 (LRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-10190
EDTA Plasma (n=5)78-9282
Heparin Plasma (n=5)96-10399
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lrg1 Microplate96 Wells (12 x 8 Well Strips)
Lrg1 Lyophilized Standard2 x 1,000 ng
100X Biotinylated Lrg1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 6.3 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). No significant cross-reactivity or interference between Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLrg1
Gene Full Nameleucine-rich alpha-2-glycoprotein 1
Alias Symbols1300008B03Rik;2310031E04Rik;leucine-rich alpha-2-glycoprotein;Lr;Lrg;Lrhg.
NCBI Gene Id76905
Protein NameLeucine-rich HEV glycoprotein
Description of TargetLeucine-rich HEV glycoprotein
Uniprot IDQ91XL1
Protein Accession #NP_084072.1
Nucleotide Accession #NM_029796.2
Protein Size (# AA)342
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