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Catalog No: OKCD00232 (Formerly GWB-KBBTJ6)
Size:96WELLS
Price: $625.00
SKU
OKCD00232
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Datasheets/ManualsPrintable datasheet for LPO ELISA Kit (Mouse) (OKCD00232)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
89-98%92-101%78-103%78-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoperoxidase (LPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactoperoxidase (LPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoperoxidase (LPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lactoperoxidase (LPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
81-9184
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lpo Microplate96 Wells (12 x 8 Well Strips)
Lpo Lyophilized Standard2 x 10 ng
100X Biotinylated Lpo Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeBreast milk, tear and other biological fluids.
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lactoperoxidase (LPO). No significant cross-reactivity or interference between Lactoperoxidase (LPO) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLpo
Gene Full Namelactoperoxidase
Alias Symbols5830499B15Rik;lactoperoxidase.
NCBI Gene Id76113
Protein NameLactoperoxidase
Description of TargetLactoperoxidase
Uniprot IDQ5SW46
Protein Accession #NP_536345
Nucleotide Accession #NM_080420.2
Protein Size (# AA)710
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