LPO ELISA Kit (Bovine) (OKCD06221)

Catalog No: OKCD06221

Size:96WELLS

LPO ELISA Kit (Bovine) (OKCD06221) Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoperoxidase (LPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactoperoxidase (LPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoperoxidase (LPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lactoperoxidase (LPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LPO Microplate: 96 Wells (12 x 8 Well strips)
  • LPO Lyophilized Standard: 2
  • 100X LPO HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Milk, tear and other biological fluids
• Sensitivity: < 0.055ng/mL.

Target Info

• Gene Symbol: LPO
• Gene Full Name: lactoperoxidase
• Alias Symbols: lactoperoxidase;lactoperoxydase.
• NCBI Gene Id: 280844
• Protein Name: Lactoperoxidase
• Description of Target: Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). May protect the udder from infection and promote growth in newborn calves. Inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.
• Uniprot ID: P80025
• Protein Accession #: NP_776358.1
• Nucleotide Accession #: NM_173933.2