LPIN1 Antibody - C-terminal region (OAGA03088)

Catalog No: OAGA03088

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
diluted at 1:1000

LPIN1 antibody [C3], C-term detects LPIN1 protein at cytosol and nucleus on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
LPIN1 antibody [C3], C-term dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lipin 1 antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Lipin 1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: LPIN1
• Gene Full Name: lipin 1
• Alias Symbols: PAP1
• NCBI Gene Id: 23175
• Protein Name: phosphatidate phosphatase LPIN1
• Description of Target: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes.
• Uniprot ID: Q14693
• Protein Accession #: NP_001248356.1
• Nucleotide Accession #: NM_001261427.1