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Catalog No: OKCD02702
Size:96WELLS
Price: $700.00
SKU
OKCD02702
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Datasheets/ManualsPrintable datasheet for LPCAT2 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 2 (LPCAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 2 (LPCAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 2 (LPCAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LPCAT2 Microplate96 Wells (12 x 8 Well strips)
LPCAT2 Lyophilized Standard2
100X Biotinylated LPCAT2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 2 (LPCAT2). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLpcat2
Gene Full Namelysophosphatidylcholine acyltransferase 2
Alias Symbols1-acylglycerol-3-phosphate O-acyltransferase 11;1-acylglycerophosphocholine O-acyltransferase;1-AGP acyltransferase 11;1-AGPAT 11;1-alkenylglycerophosphocholine O-acyltransferase;1-alkylglycerophosphocholine O-acetyltransferase;A330042H22;acetyl-CoA:lyso-PAF acetyltransferase;acetyl-CoA:lyso-platelet-activating factor acetyltransferase;acyl-CoA:lysophosphatidylcholine acyltransferase member 1;acyltransferase like 1A;Acyltransferase-like 1;Ayt;Aytl1;Aytl1a;lpafat1;LPC;LPC acyltransferase 2;lyso-PAF acetyltransferase;lysoPAFAT;lysoPAFAT/LPCAT2;lysoPC acyltransferase 2;lysophosphatidylcholine acyltransferase 2.
NCBI Gene Id270084
Protein NameQ8BYI6
Description of TargetLysophosphatidylcholine acyltransferase 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q8BYI6
Protein Accession #NP_766602.1
Nucleotide Accession #NM_173014.1
Protein Size (# AA)544
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