Catalog No: OKCD08952
Size:96WELLS
Price: $825.00
SKU
OKCD08952
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for LPCAT1 ELISA Kit (Mouse) (OKCD08952) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 1 (LPCAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.056ng/mL |
Gene Symbol | Lpcat1 |
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Gene Full Name | lysophosphatidylcholine acyltransferase 1 |
Alias Symbols | 1-acylglycerol-3-phosphate O-acyltransferase;1-acylglycerophosphocholine O-acyltransferase;1-alkenylglycerophosphocholine O-acyltransferase;1-alkylglycerophosphocholine O-acetyltransferase;2900035H07Rik;acetyl-CoA:lyso-PAF acetyltransferase;acetyl-CoA:lyso-platelet-activating factor acetyltransferase;acyltransferase-like 2;Ayt;Aytl2;BB137372;BC005662;C87117;LP;LPC acyltransferase 1;LPCAT;LPCAT-1;lyso-PAF acetyltransferase;lysoPAFAT;lysoPC acyltransferase 1;lysophosphatidylcholine acyltransferase 1;mLPCAT1;rd1;rd11. |
NCBI Gene Id | 210992 |
Protein Name | Lysophosphatidylcholine acyltransferase 1 |
Description of Target | Possesses both acyltransferase and acetyltransferase activities (PubMed:16704971). Activity is calcium-independent (PubMed:18285344). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:16704971, PubMed:18156367). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:16704971, PubMed:18285344). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16704971). Involved in the regulation of lipid droplet number and size (By similarity). |
Uniprot ID | Q3TFD2 |
Protein Accession # | NP_663351.3 |
Nucleotide Accession # | NM_145376.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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