LOXL4 ELISA Kit (Human) (OKCD02701)

Catalog No: OKCD02701

Size:96WELLS

Human LOXL4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-96%	78-94%	96-104%	90-99%
  EDTA Plasma (n=5)	86-97%	88-102%	98-105%	81-93%
  Heparin Plasma (n=5)	86-94%	82-96%	80-93%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase Like Protein 4 (LOXL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase Like Protein 4 (LOXL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase Like Protein 4 (LOXL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl Oxidase Like Protein 4 (LOXL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-89	82
  EDTA Plasma (n=5)	84-94	90
  Heparin Plasma(n=5)	79-91	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 4 (LOXL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 4 (LOXL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LOXL4 Microplate: 96 Wells (12 x 8 Well strips)
  • LOXL4 Lyophilized Standard: 2
  • 100X Biotinylated LOXL4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase Like Protein 4 (LOXL4). No significant cross-reactivity or interference between Lysyl Oxidase Like Protein 4 (LOXL4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LOXL4
• Gene Full Name: lysyl oxidase like 4
• Alias Symbols: LOXC;lysyl oxidase homolog 4;lysyl oxidase related C;lysyl oxidase-like 4 pseudogene;lysyl oxidase-like protein 4;lysyl oxidase-related protein C.
• NCBI Gene Id: 84171
• Protein Name: Lysyl oxidase homolog 4
• Description of Target: May modulate the formation of a collagenous extracellular matrix.
• Uniprot ID: Q96JB6
• Protein Accession #: NP_115587.6
• Nucleotide Accession #: NM_032211.6
• Protein Size (# AA): 756