LOXL1 ELISA Kit (Mouse) (OKCD02699)

Catalog No: OKCD02699

Size:96WELLS

Mouse LOXL1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	95-104%	79-103%	96-104%
  EDTA Plasma (n=5)	78-96%	79-103%	88-101%	91-98%
  Heparin Plasma (n=5)	91-101%	91-99%	85-96%	94-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase Like Protein 1 (LOXL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase Like Protein 1 (LOXL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase Like Protein 1 (LOXL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl Oxidase Like Protein 1 (LOXL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-102	91
  EDTA Plasma (n=5)	79-98	94
  Heparin Plasma(n=5)	84-96	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 1 (LOXL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 1 (LOXL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LOXL1 Microplate: 96 Wells (12 x 8 Well strips)
  • LOXL1 Lyophilized Standard: 2
  • 100X Biotinylated LOXL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 31 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase Like Protein 1 (LOXL1). No significant cross-reactivity or interference between Lysyl Oxidase Like Protein 1 (LOXL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Loxl1
• Gene Full Name: lysyl oxidase-like 1
• Alias Symbols: L;Loxl;lysyl oxidase 2;lysyl oxidase homolog 1;lysyl oxidase-like protein 1.
• NCBI Gene Id: 16949
• Protein Name: Lysyl oxidase homolog 1
• Description of Target: Active on elastin and collagen substrates.
• Uniprot ID: P97873
• Protein Accession #: NP_034859.2
• Nucleotide Accession #: NM_010729.3
• Protein Size (# AA): 607