LMNB2 Antibody (OAGA00804)

Catalog No: OAGA00804

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
7.5% SDS PAGE
OAGA00804 diluted at 1:1000

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 lysate/extract
F. 30 ug Raw264.7 whole cell lysate/extract
G. 30 ug C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin B2 antibody [N3C2], Internal (OAGA00804) dilution: 1:500

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [N3C2], Internal (OAGA00804) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]
  Positive Controls: H1299, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12
  Cellular Localization: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 221 and 500 of Lamin B2 (Uniprot ID#Q03252)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: LMNB2
• Gene Full Name: Lamin-B2
• Alias Symbols: EPM9, LMN2, LAMB2, MCPH27
• NCBI Gene Id: 84823
• Protein Name: Lamin-B2
• Uniprot ID: Q03252
• Protein Accession #: NP_116126.3
• Molecular Weight: 68 kDa