LMNB1 Antibody (OABB00589)

Catalog No: OABB00589

Size:100UG

Lane1: Hela Cell Lysate
Lane2: U87 Cell Lysate

IHC(P):Rat Intestines Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Immunohistochemistry-Frozen|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons. Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, is generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.
  Background: Lamin B2,also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons.Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2,is generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Rat: Human, Mouse
• Reference: 1. Biamonti, G., Giacca, M., Perini, G., Contreas, G., Zentilin, L., Weighardt, F., Guerra, M., Della Valle,
  G., Saccone, S., Riva, S., Falaschi, A. The gene for a novel human lamin maps at a highly
  transcribed locus of chromosome 19 which replicates at the onset of S-phase. Molec. Cell. Biol.
  2. Giacca, M., Zentilin, L., Norio, P., Diviacco, S., Dimitrova, D., Contreas, G., Biamonti, G., Perini, G.,
  Weighardt, F., Riva, S., Falaschi, A. Fine mapping of a replication origin of human DNA. Proc. Nat.
  Acad. Sci. 91: 7119-7123, 1994
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Lamin-B2(LMNB2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Target Info

• Gene Symbol: LMNB2
• Gene Full Name: lamin B2
• Alias Symbols: epididymis secretory sperm binding protein;EPM9;LAMB2;lamin B3;lamin-B2;LMN2;MCPH27.
• NCBI Gene Id: 84823
• Protein Name: Lamin-B2
• Description of Target: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
• Uniprot ID: Q03252
• Protein Accession #: NP_005564.1
• Nucleotide Accession #: NM_005573.3
• Molecular Weight: 67689 MW