LMNB1 Antibody - middle region (OAEB03011)

Catalog No: OAEB03011 (Formerly GWB-EV1425)

Size:100UG

OAEB03011 (1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: LMNB1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Dog, Pig, Cow
• Peptide Sequence: C-DVKVILKNSQGE
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).
• Epitope: middle region (near C-terminal region)
• Application Info: P-ELISA: Antibody detection limit dilution 1:4000
  WB: Approx 70 kDa band observed in nuclear lysates of cell lines HeLa and Jurkat and approx. 70-75 kDa in Mouse Liver lysates (calculated MW of 66.4 kDa according to Human NP_005564.1 and 66.8 kDa according to Mouse NP_034851.2) Recommended concentration: 1-3 ug/ml.

Target Info

• Gene Symbol: Lmnb1|LMNB1
• Gene Full Name: lamin B1
• Alias Symbols: ADLD;lamin-B1;LMN;LMN2;LMNB;MCPH26.
• NCBI Gene Id: 16906|4001
• Protein Accession #: NP_005564.1;
  NP_001185486.1
• Molecular Weight: 66.4; 43.0