Lipoxin B4 ELISA Kit (OKEH02620)

Catalog No: OKEH02620

Size:96T

Lipoxin B4 ELISA Kit (OKEH02620) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Lipoxin B4 ELISA Kit (OKEH02620) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Lipoxin B4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lipoxin B4 and incubated. The Lipoxin B4 found in the sample or standards competes with the biotinylated Lipoxin B4 for limited binding sites on the immobilized anti-Lipoxin B4 antibody. Excess unbound biotinylated Lipoxin B4 and sample or standard Lipoxin B4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lipoxin B4 captured in the well and inversely proportional to the amount of Lipoxin B4 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.6% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-Lipoxin B4 Microplate: 96 Wells (12 x 8 Well strips)
  • Lipoxin B4 Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Lipoxin B4-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: LXB4
  Target Name: Lipoxin B4
  Pubchem: 5280915
  Chemical formula: C20H32O5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.33 ng/mL
• Specificity: Natural and recombinant General Lipoxin B4
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: LXB4, Lipoxin B4, LXB4
• Protein Name: Lipoxin B4
• Description of Target: Lipoxins are a series of anti-inflammatory mediators. Lipoxins are short lived endogenously produced nonclassic eicosanoids whose appearance in inflammation signals the resolution of inflammation. They are abbreviated as LX, an acronym for lipoxygenase (LO) interaction products. At present two lipoxins have been identified; lipoxin A4 (LXA4) and lipoxin B4 (LXB4).