Lipopolysaccharide ELISA Kit (OKEH02594)

Catalog No: OKEH02594

Size:96T

Lipopolysaccharide ELISA Kit (OKEH02594) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Lipopolysaccharide ELISA Kit (OKEH02594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Lipopolysaccharide has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lipopolysaccharide is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lipopolysaccharide captured in the well.
• ELISA Kit Range: 7.8-500 pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.4% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-Lipopolysaccharide Microplate: 96 Wells (12 x 8 Well strips)
  • Lipopolysaccharide Lyophilized Standard: 2 x 5 EU
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Lipopolysaccharide Detector Ab: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: LPS
  Target Name: Lipopolysaccharide
  Pubchem: 11970143
  Chemical formula: C205H366N3O117P5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 3.5 pg/mL
• Specificity: Natural and recombinant General Lipopolysaccharide
• Publications:

  Rhubarb Enema Improved Colon Mucosal Barrier Injury in 5/6 Nephrectomy Rats May Associate With Gut Microbiota Modification. Front Pharmacol. 11, 1092 (2020). 32848732

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for Lipopolysaccharide ELISA Kit (OKEH02594) on CiteAb

Target Info

• Alias Symbols: LPS, Lipopolysaccharide, LPS
• Protein Name: Lipopolysaccharide
• Description of Target: Lipopolysaccharides (LPS), also known as lipoglycans, are large molecules consisting of a lipid and a polysaccharide joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, act as endotoxins and elicit strong immune responses in animals.