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Catalog No: OKCD02692
Size:96WELLS
Price: $650.00
SKU
OKCD02692
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Datasheets/ManualsPrintable datasheet for LIPE ELISA kit (Human) (OKCD02692)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-103%89-97%80-93%79-90%
EDTA Plasma (n=5)82-99%96-105%82-89%88-96%
Heparin Plasma (n=5)98-105%95-102%87-94%90-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hormone Sensitive (LIPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hormone Sensitive (LIPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hormone Sensitive (LIPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase, Hormone Sensitive (LIPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-10498
EDTA Plasma (n=5)92-10197
Heparin Plasma(n=5)84-10595
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LIPE Microplate96 Wells (12 x 8 Well strips)
LIPE Lyophilized Standard2
100X Biotinylated LIPE Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Hormone Sensitive (LIPE). No significant cross-reactivity or interference between Lipase, Hormone Sensitive (LIPE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLIPE
Gene Full Namelipase E, hormone sensitive type
Alias SymbolsAOMS4;FPLD6;hormone-sensitive lipase;HSL;LHS;lipase, hormone-sensitive;monoacylglycerol lipase LIPE;REH;retinyl ester hydrolase.
NCBI Gene Id3991
Protein NameHormone-sensitive lipase
Description of TargetIn adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Uniprot IDQ05469
Protein Accession #NP_005348.2
Nucleotide Accession #NM_005357.3
Protein Size (# AA)1076
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