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Datasheets/Manuals | Printable datasheet for OAAB11191 |
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Predicted Species Reactivity | Human |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IF, IHC, FC |
Additional Information | Precautions: LIN28B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth |
:: | Cellular Location: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively Tissue Location: High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level) |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | IF: 1:25 FC: 1:25 WB: 1:2000 WB: 1:1000 |
Gene Symbol | LIN28B |
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Alias Symbols | CSDD2 |
NCBI Gene Id | 389421 |
Protein Name | Protein lin-28 homolog B |
Description of Target | Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | Q6ZN17 |
Protein Accession # | NP_001004317.1 |
Molecular Weight | 27084 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LIN28B Antibody - N-terminal region (OAAB11191)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "LIN28B Antibody - N-terminal region (OAAB11191)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "LIN28B Antibody - N-terminal region (OAAB11191)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LIN28B Antibody - N-terminal region (OAAB11191)"?
This target may also be called "CSDD2" in publications.
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What is the shipping cost for "LIN28B Antibody - N-terminal region (OAAB11191)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LIN28B Antibody - N-terminal region (OAAB11191)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LIN28B Antibody - N-terminal region (OAAB11191)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "27084 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LIN28B Antibody - N-terminal region (OAAB11191)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LIN28B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LIN28B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LIN28B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LIN28B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LIN28B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LIN28B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.