LIN28A Antibody (OAAB06652)

Catalog No: OAAB06652

Size:400ul

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 55CT58.12.1
• Isotype: IgG1 Kappa
• Host: Mouse
• Application: WB, IHC, IF
• Additional Information: Precautions: LIN28A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7 Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation
  Cellular Location: Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules
  Tissue Location: Expressed in embryonic stem cells (ES cells), placenta and testis
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: LIN28A recombinant protein is used to produce this monoclonal antibody.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:200~2000
  IHC-P: 1:50~200
  IF: 1:10~50

Target Info

• Gene Symbol: LIN28A
• Alias Symbols: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
• NCBI Gene Id: 79727
• Protein Name: Protein lin-28 homolog A
• Description of Target: Purified Mouse Monoclonal Antibody (Mab)
• Uniprot ID: Q9H9Z2
• Protein Accession #: NP_078950.1
• Molecular Weight: 22743 Da