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Catalog No: OPPA02194 (Formerly GWB-F7C577)
Size:2UG
Price: $75.00
SKU
OPPA02194
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LIN28 Protein (OPPA02194)

Datasheets/ManualsPrintable datasheet for OPPA02194
Product Info
Predicted Species ReactivityHuman
Product FormatThe LIN28 protein solution contains 20mM Tris-HCl, pH-8, 10% glycerol and 0.1M NaCl. Physical appearance: Sterile filtered colorless solution.
HostE. Coli
::Product Introduction: LIN28 plays an important role as a 'translational enhancer', leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 is a marker of undifferentiated human embryonic stem cells and it enhances the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 binds to the let-7 pre-miRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. Overexpression of LIN28 is associated with human germ-cell tumors.
Product Description: Recombinant Human LIN28 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids (42-209) and having a molecular mass of 21.1 kDa.LIN28 is expressed with a 23 amino acid His tag fused at N-Terminus and purified by proprietary chromatographic techniques.
Reconstitution and StorageStore at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Avoid multiple freeze-thaw cycles.
PurityGreater than 90.0% as determined by SDS-PAGE
Peptide SequenceMGSSHHHHHH SSGLVPRGSHRSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N.
Gene SymbolLIN28
Alias SymbolsCSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
NCBI Gene Id79727
Protein NameProtein lin-28 homolog A
Description of TargetRecombinant Human LIN28
Uniprot IDQ9H9Z2
Protein Accession #NP_078950.1
Protein Size (# AA)Recombinant
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