LIMS1 ELISA Kit (Human) (OKCD00634)

Catalog No: OKCD00634 (Formerly GWB-KBBUC3)

Size:96WELLS

Human LIMS1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LIMS1 Microplate: 96 Wells (12 x 8 Well Strips)
  • LIMS1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated LIMS1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). No significant cross-reactivity or interference between LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LIMS1
• Gene Full Name: LIM zinc finger domain containing 1
• Alias Symbols: LIM and senescent cell antigen-like domains 1;LIM and senescent cell antigen-like-containing domain protein 1;LIM-type zinc finger domains 1;particularly interesting new Cys-His protein 1;PINCH;PINCH1;PINCH-1;renal carcinoma antigen NY-REN-48;senescent cell antigen.
• NCBI Gene Id: 3987
• Protein Name: LIM and senescent cell antigen-like-containing domain protein 1
• Description of Target: Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
• Uniprot ID: P48059
• Protein Accession #: NP_001180411
• Nucleotide Accession #: NM_001193482.1
• Protein Size (# AA): 325