LIME1 Antibody (OAEE01115)

Catalog No: OAEE01115

Size:100UG

Immunohistochemistry staining of human spleen (paraffin sections) with anti-LIME polyclonal antibody.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Polyclonal
• Host: Rabbit
• Application: Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Reference: Brdickova N, Brdicka T, Angelisova P, Horvath O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsi V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
  Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
  Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
  Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Target Info

• Gene Symbol: LIME1
• Gene Full Name: Lck interacting transmembrane adaptor 1
• Alias Symbols: dJ583P15.4;lck-interacting membrane protein;lck-interacting molecule;lck-interacting transmembrane adapter 1;LIME.
• NCBI Gene Id: 54923
• Protein Name: Lck-interacting transmembrane adapter 1
• Description of Target: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
• Uniprot ID: Q9H400
• Protein Accession #: NP_060276.2
• Nucleotide Accession #: NM_017806.3