LILRB1 Antibody (OAEE00325)

Catalog No: OAEE00325

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4
• Clonality: Monoclonal
• Clone: GHI/75
• Isotype: IgG2b
• Host: Mouse
• Application: Flow cytometry|Functional assay|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Hairy cell leukaemia cells
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The mouse monoclonal antibody GHI/75 recognizes CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
• Formulation: Low endotoxin
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Reference: Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb;13(2):193-201.
  Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun;65(6):841-5.
  

Target Info

• Gene Symbol: LILRB1
• Gene Full Name: leukocyte immunoglobulin like receptor B1
• Alias Symbols: CD85 antigen-like family member J;CD85J;Ig-like transcript 2;ILT2;ILT-2;immunoglobulin heavy chain variable region;Immunoglobulin-like transcript 2;leucocyte Ig-like receptor B1;leukocyte immunoglobulin-like receptor subfamily B member 1;leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1;LIR1;LIR-1;MIR7;MIR-7;monocyte/macrophage immunoglobulin-like receptor 7;myeloid inhibitory receptor 7;PIRB;PIR-B.
• NCBI Gene Id: 10859
• Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• Description of Target: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
• Uniprot ID: Q8NHL6
• Protein Accession #: NP_001075106.2; NP_001075107.2; NP_001075108.2; NP_001265327.2; NP_001265328.2; NP_006660.4
• Nucleotide Accession #: NM_001081637.2; NM_001081638.3; NM_001081639.3; NM_001278398.2; NM_001278399.2; NM_006669.6