Catalog No: OOMB00002
Size:6mL
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LightOn Anti-Fade Mounting Media for Immunofluorescence ICC & IHC (OOMB00002)
Datasheets/Manuals | Printable datasheet for OOMB00002 |
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Product Format | Liquid containing glycerol, which is a viscous reagent. To facilitate its aspiration and dispensing, it is recommended to trim the ends of pipette tips to make wider opening. |
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Application | ICC, IHC |
Reconstitution and Storage | LightOn media has to be stored in a freezer at -20°C or below. To use the medium, remove the vial from the freezer and warm it up by keeping it for 5-7 minutes at room temperature. Avoid exposing LightOn medium to direct bright light. After mounting stained tissue sections on histological slides, store them in a freezer in a horizontal position to avoid leakage of mounting medium, which does not harden over time and remains liquid. LightOn is stable for up to one year when stored at -20°C. Mounted stain specimens retain their fluorescence for up to one year when stored in a freezer at -20°C, protected from light. |
Application Info | LightOn can be used for both IHC and ICC on tissue sections/cytosmears and fixed cells in culture chamber slides. LightOn medium can be used either "as-is" or mixed with DAPI and similar cell nuclei fluorescence counterstains. |
Warning | Use PPE when working with LightOn and avoid getting it into the mouth, eyes, and on the skin. |
Description of Target | LightOn is a ready to use glycerol-based mounting medium. |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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