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Catalog No: OKCD02689
Size:96WELLS
Price: $625.00
SKU
OKCD02689
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Datasheets/ManualsPrintable datasheet for LIFR ELISA Kit (Mouse) (OKCD02689)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)88-102%79-102%80-95%78-96%
EDTA Plasma (n=5)87-94%91-101%82-96%80-101%
Heparin Plasma (n=5)92-101%94-101%87-101%93-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor Receptor (LIFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukemia Inhibitory Factor Receptor (LIFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9692
EDTA Plasma (n=5)82-9787
Heparin Plasma(n=5)80-9083
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukemia Inhibitory Factor Receptor (LIFR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukemia Inhibitory Factor Receptor (LIFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LIFR Microplate96 Wells (12 x 8 Well strips)
LIFR Lyophilized Standard2
100X Biotinylated LIFR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity31 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leukemia Inhibitory Factor Receptor (LIFR). No significant cross-reactivity or interference between Leukemia Inhibitory Factor Receptor (LIFR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLifr
Gene Full NameLIF receptor alpha
Alias SymbolsA230075M04Rik;AW061234;D-factor/LIF receptor;leukemia inhibitory factor receptor;leukemia inhibitory factor receptor alpha chain;LIF;LIF-R;soluble differentiation-stimulating factor receptor.
NCBI Gene Id16880
Protein NameLeukemia inhibitory factor receptor
Description of TargetSignal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
Uniprot IDP42703
Protein Accession #NP_001106857.1
Nucleotide Accession #NM_001113386.1
Protein Size (# AA)1092
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