Catalog No: OPPA02003 (Formerly GWB-6D0754)
Size:2ug
Price: $103.00
SKU
OPPA02003
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LIF Protein (OPPA02003)

Datasheets/ManualsPrintable datasheet for OPPA02003
Product Info
Product FormatLyophilized from a concentrated solution in water containing 1x PBS, pH 7.4.
Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution and StorageIt is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PurificationThe Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
PurityGreater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological ActivityThe ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100,000,000 IU/mg.
Peptide SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Leu-Pro-Ile.
SourceE.coli
Gene SymbolLIF
Alias SymbolsCDF, DIA, HILDA, MLPLI
NCBI Gene Id3976
Protein NameLeukemia inhibitory factor
Description of TargetLeukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids.
Uniprot IDP15018
Protein Accession #NP_002300.1
Protein Size (# AA)180
Molecular Weight20 kDa
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