Catalog No: OPPA02003 (Formerly GWB-6D0754)
Size:2ug
Price: $103.00
SKU
OPPA02003
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA02003 |
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Product Format | Lyophilized from a concentrated solution in water containing 1x PBS, pH 7.4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
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Reconstitution and Storage | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purification | The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
Purity | Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Biological Activity | The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100,000,000 IU/mg. |
Peptide Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Leu-Pro-Ile. |
Source | E.coli |
Gene Symbol | LIF |
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Alias Symbols | CDF, DIA, HILDA, MLPLI |
NCBI Gene Id | 3976 |
Protein Name | Leukemia inhibitory factor |
Description of Target | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids. |
Uniprot ID | P15018 |
Protein Accession # | NP_002300.1 |
Protein Size (# AA) | 180 |
Molecular Weight | 20 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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