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LIF ELISA Kit (Rat) (OKEH03028)

96 Wells
$529.00
In Stock
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Gene Symbol:
LIF
Official Gene Full Name:
leukemia inhibitory factor
NCBI Gene Id:
60584
Alias Symbols:
Cholinergic neuronal differentiation factor, Leukemia inhibitory factor, LIF
Description of Target:
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Protein Name:
Leukemia inhibitory factor
Swissprot Id:
P17777
Protein Accession #:
NP_071532.2
Nucleotide Accession #:
NM_022196.2 
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
39 pg/mL
Kit Range:
78 - 5,000 pg/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology LIF ELISA Kit (Rat) (OKEH03028) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lif has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lif is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lif captured in well.
Kit Component:
ComponentAmount
Anti-Lif Microplate96 Wells (12 x 8 Well strips)
Lif Lyophilized Standard2
100X Biotinylated Lif Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Lif.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Lif.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat LIF. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
22 kDa
Protein Size (# AA):
202

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