Size:96 Wells
$469.00
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SKU
OKEH03028
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LIF ELISA Kit (Rat) (OKEH03028)

Catalog#: OKEH03028
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology LIF ELISA Kit (Rat) (OKEH03028) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lif has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lif is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lif captured in well.
Kit Range78 - 5000pg/mL
Kit Component
ComponentAmount
Anti-Lif Microplate96 Wells (12 x 8 Well strips)
Lif Lyophilized Standard2
100X Biotinylated Lif Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.5 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Rat Leukemia inhibitory factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLIF
Official Gene Full Nameleukemia inhibitory factor
Alias SymbolsCholinergic neuronal differentiation factor, Leukemia inhibitory factor, LIF
NCBI Gene Id60584
Protein NameLeukemia inhibitory factor
Description of TargetLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Swissprot IdP17777
Protein Accession #NP_071532.2
Nucleotide Accession #NM_022196.2 
Protein Size (# AA)202
Molecular Weight22 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Lif.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Lif.
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