LIF ELISA Kit (Mouse) (OKBB00760)

Catalog No: OKBB00760

Size:96 wells

LIF ELISA Kit (Mouse) (OKBB00760) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse LIF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LIF has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: S24-F203) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LIF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse LIF amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	16	70	272	15	76	278
  Standard deviation	0.7	3.01	19.58	0.72	3.34	24.74
  CV(%)	4.4	4.3	7.2	4.8	4.4	8.9

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse LIF Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse LIF Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse LIF Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	16	15	16	15	15	0.5	3.3%
  Sample 2	70	65	74	67	69	3.39	4.9%
  Sample 3	272	305	287	293	289	11.88	4.1%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
  O.D.	0.008	0.062	0.141	0.295	0.574	1.100	1.981	2.761

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse LIF
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S24-F203
• Description: For quantitative detection of mouse LIF in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: LIF
• Gene Full Name: leukemia inhibitory factor
• Alias Symbols: CDF, Cholinergic differentiation factor, D factor, DIA, Differentiation inhibitory activity, Differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, Hepatocyte stimulating factor III, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, Melanoma-derived LPL inhibitor, MLPL I, P15018
• NCBI Gene Id: 16878
• Protein Name: Leukemia inhibitory factor
• Description of Target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.
• Uniprot ID: P09056
• Protein Accession #: NP_001034626.1
• Nucleotide Accession #: NM_001039537.2