LGR5/GPR49 Antibody - N-terminal region (OAAB15973)

Catalog No: OAAB15973

Size:400ul

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
  Cellular Location: Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven.
  Tissue Location: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 0.5 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:500

Target Info

• Gene Symbol: LGR5
• Alias Symbols: FEX, HG38, GPR49, GPR67, GRP49
• NCBI Gene Id: 8549
• Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Description of Target: LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
• Uniprot ID: O75473
• Protein Accession #: NP_003658.1
• Molecular Weight: 100 kDa