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Datasheets/Manuals | Printable datasheet for OAAB15976 |
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Predicted Species Reactivity | Human, Cow |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC, FC |
Additional Information | Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
:: | Cellular Location: Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653) Tissue Location: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49). |
Predicted Homology Based on Immunogen Sequence | Cow; Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Sample Type Confirmation | LGR5 is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
Intended Use | LGR5 (GPR49) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 IHC-P: 1:10~50 FC: 1:10~50 |
Gene Symbol | LGR5 |
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Alias Symbols | FEX, HG38, GPR49, GPR67, GRP49 |
NCBI Gene Id | 8549 |
Protein Name | Leucine-rich repeat-containing G-protein coupled receptor 5 |
Description of Target | Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | O75473 |
Protein Accession # | NP_003658.1 |
Molecular Weight | 99998 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LGR5 Antibody - middle region (OAAB15976)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Cow".
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How long will it take to receive "LGR5 Antibody - middle region (OAAB15976)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "LGR5 Antibody - middle region (OAAB15976)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LGR5 Antibody - middle region (OAAB15976)"?
This target may also be called "FEX, HG38, GPR49, GPR67, GRP49" in publications.
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What is the shipping cost for "LGR5 Antibody - middle region (OAAB15976)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LGR5 Antibody - middle region (OAAB15976)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LGR5 Antibody - middle region (OAAB15976)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "99998 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LGR5 Antibody - middle region (OAAB15976)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LGR5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LGR5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LGR5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LGR5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LGR5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LGR5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.