LGP2 Antibody (OAPB00582)

Catalog No: OAPB00582

Size:100UG

Western blot analysis of LGP2 in rat kidney tissue lysate with LGP2 antibody at (A) 1 and (B) 2 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. LGP2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: LGP2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: LGP2 antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human LGP2. The immunogen is located within amino acids 570 - 620 of LGP2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-LGP2 antibody is Catalog # OEPB02699
• Application Info: LGP2 antibody can be used for detection of LGP2 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell2006; 124:783-801.;Iwasaki A and Medzhitov R. Toll-like receptor control of the adaptive immune responses. Nat. Immunol.2004; 5:987-95.;Yoneyama M, Kikuchi K, Matsumoto K, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.2004; 5:730-7.;Andrejeva J, Childs KS, Young DF, et al. The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibits its activation of the IFN-betta promoter. Proc. Natl. Acad. Sci. USA, 2004; 101:17264-9.

Target Info

• Gene Symbol: DHX58
• Gene Full Name: DExH-box helicase 58
• Alias Symbols: D11LGP2;D11lgp2e;DEXH (Asp-Glu-X-His) box polypeptide 58;LGP2;ortholog of mouse D11lgp2;probable ATP-dependent helicase LGP2;probable ATP-dependent RNA helicase DHX58;protein D11Lgp2 homolog;RIG-I-like receptor 3;RIG-I-like receptor LGP2;RLR;RLR-3;RNA helicase LGP2.
• NCBI Gene Id: 79132
• Protein Name: likely ortholog of mouse D11lgp2, isoform CRA_b [Homo sapiens]|Probable ATP-dependent RNA helicase DHX58
• Description of Target: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.
• Uniprot ID: Q96C10
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/EAW60798
• Molecular Weight: Predicted: 70 kDa Observed: 75 kDa