LGI1 Antibody : HRP (OASE00307-HRP)

Catalog No: OASE00307-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S283-7
• Isotype: IgG2a
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, ICC, IHC
• Additional Information: Background Info: Detects a 60 kDa protein corresponding to the molecular weight of LGI1.
  Scientific Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.
  Certificate of Analysis: 1ug/mL was sufficient for detection of LGI1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1, accession number Q9JIA1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Reference: 1. Chernova O.B., Somerville R.P. and Cowell J.K. (1998) Oncogene. 17:2873-81.
  2. Fialka F., et al. (2008) Oral Oncol.
  3. Gu W., et al. (2005) Mol. Biol. Evol. 22:2209-16.
  4. Fukata Y., et al. (2006) Science. 313:1792-5.
  

Target Info

• Gene Symbol: Lgi1
• Gene Full Name: leucine-rich repeat LGI family, member 1
• Alias Symbols: BB130740
• NCBI Gene Id: 56839
• Uniprot ID: Q9JIA1.1
• Protein Accession #: NP_064674.1