Catalog No: OKEH03893
Size:96T
Price: $625.00
SKU
OKEH03893
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LGB ELISA Kit (Bovine) (OKEH03893) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGB captured in well.
ELISA Kit Range1.56-100ug/mL
ELISA Kit RecoveryAverage recovery when spiking into serum: 106%
ELISA Kit ReproducibilityMean Intra-Assay CV: <6.1% (n=20)
Inter-Assay CV: <9.9% (n=24)
ELISA Kit Component
ComponentAmount
Anti-LGB Microplate96 Wells (12 x 8 Well strips)
LGB Lyophilized Standard2
100X Biotinylated LGB Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.47ug/mL
SpecificityNatural and recombinant Bovine Beta-lactoglobulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPAEP
Gene Full Nameprogestagen-associated endometrial protein
Alias Symbolsbeta lactoglobulin C;beta lactoglobulin D;beta-lactoglobulin;beta-LG;BLG;bLG C;lactoglobulin, beta;LGB.
NCBI Gene Id280838
Protein NameP02754
Description of TargetBeta-lactoglobulin
Uniprot IDhttps://www.uniprot.org/uniprot/P02754
Protein Accession #NP_776354.2
Nucleotide Accession #NM_173929.3
Protein Size (# AA)178
Molecular Weight20 kDa
Write Your Own Review
You're reviewing:LGB ELISA Kit (Bovine) (OKEH03893)
Your Rating
We found other products you might like!