LGALS8 Protein (OOPA00033)

Catalog No: OOPA00033 (Formerly GWB-BSP027)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: Escherichia Coli.
• Additional Information: Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized Galectin-8 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of the galectin family, consisting of 2 CRDs attached by a linker peptide. Galectin-8 is greatly expressed in lung carcinomas, a number of forms of prostate carcinomas, in addition to other tumor cells. Galectin-8 attaches to a subset of cell surface integrins to modulate ECM-integrin interactions. Once immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. On the other hand, as a soluble ligand, Galectin-8 can inhibit cell adhesion.
  Product Description: Galectin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 317 amino acids and having a molecular mass of 35.8kDa. The LGALS8 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized LGALS8 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Galectin-8 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
• Formulation: LGALS8 was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4.
• Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The ED50 of Galectin-8 as determined by its ability to agglutinate human red blood cells is 0.8~4 ug/ml.
• Peptide Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Met-Leu-Ser-Leu.

Target Info

• Gene Symbol: LGALS8
• Alias Symbols: Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Po66-CBP, Prostate carcinoma tumor antigen 1, PCTA-1, LGALS8.
• Protein Size (# AA): Recombinant