LGALS8 Antibody (OAAF03041)

Catalog No: OAAF03041 (Formerly GWB-ASE194)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, using LEG8 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using LEG8 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human LEG8.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: LEG8 antibody detects endogenous levels of total LEG8 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Su Z.-Z., Proc. Natl. Acad. Sci. U.S.A. 93:7252-7257(1996).
  Hadari Y.R., Trends Glycosci. Glycotechnol. 9:103-112(1997).
  Gopalkrishnan R.V., Oncogene 19:4405-4416(2000).

Target Info

• Gene Symbol: LGALS8
• Gene Full Name: galectin 8
• Alias Symbols: Gal-8;galectin-8;galectin-8g;lectin, galactoside-binding, soluble, 8;PCTA1;PCTA-1;Po66 carbohydrate binding protein;po66 carbohydrate-binding protein;Po66-CBP;prostate carcinoma tumor antigen 1.
• NCBI Gene Id: 3964
• Protein Name: Galectin-8
• Description of Target: Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed:22246324, PubMed:28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed:22246324, PubMed:28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed:22246324, PubMed:28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed:22246324). Also required to restrict infection of Picornaviridae viruses (PubMed:28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed:21288902).
• Uniprot ID: O00214
• Protein Accession #: NP_006490.3
• Molecular Weight: 35 kDa