LGALS7 ELISA Kit (Human) (OKCD06233)

Catalog No: OKCD06233

Size:96WELLS

LGALS7 ELISA Kit (Human) (OKCD06233) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 7 (GAL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 7 (GAL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 7 (GAL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 7 (GAL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 7 (GAL7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 7 (GAL7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LGALS7 Microplate: 96 Wells (12 x 8 Well strips)
  • LGALS7 Lyophilized Standard: 2
  • 100X LGALS7 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 12.5pg/mL

Target Info

• Gene Symbol: LGALS7|LGALS7B
• Gene Full Name: galectin 7|galectin 7B
• Alias Symbols: Gal-7;GAL7;galectin-7;HKL-14;keratinocyte lectin 14;lectin galactoside-binding soluble 7;lectin, galactoside-binding, soluble, 7;lectin, galactoside-binding, soluble, 7B;LGALS7;LGALS7A;p53-induced gene 1 protein;p53-induced protein 1;PI7.
• NCBI Gene Id: 3963|653499
• Protein Name: Galectin-7
• Description of Target: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
• Uniprot ID: P47929
• Protein Accession #: NP_001035972.1
• Nucleotide Accession #: NM_001042507.3