LGALS3BP ELISA Kit (Bovine) (OKEH08143)

Catalog No: OKEH08143

Size:96T

Bovine LGALS3BP ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology LGALS3BP ELISA Kit (Bovine) (OKEH08143) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGALS3BP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGALS3BP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGALS3BP captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.1% (n = 20)
  Mean Inter-assay CV%: <=9.0% (n = 20)
• ELISA Kit Component:
  • LGALS3BP Microplate: 96 Wells (12 x 8 Well strips)
  • LGALS3BP Lyophilized Standard: 2
  • 100X Biotinylated LGALS3BP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  Subcellular Location: Secreted Secreted Extracellular space Extracellular matrix
  Unigene: Bt.6597
  SMR: A7E3W2
  String: 9913.ENSBTAP00000001802
  KEGG: bta:531137
  Pfam: PF07707
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.15ng/mL
• Specificity: Natural and recombinant Bovine Galectin-3-binding protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LGALS3BP
• Gene Full Name: galectin 3 binding protein
• Alias Symbols: galectin-3-binding protein;lectin galactoside-binding soluble 3-binding protein.
• NCBI Gene Id: 531137
• Protein Name: A7E3W2
• Description of Target: Galectin-3-binding protein
• Uniprot ID: https://www.uniprot.org/uniprot/A7E3W2
• Protein Accession #: NP_001039781.1
• Nucleotide Accession #: NM_001046316.2